中国科学院大学存济医学院

北京市朝阳区北辰西路1号院5号
中国科学院动物研究所 干细胞与生殖生物学国家重点实验室 100101

　　周琪，博士，研究员，博士生导师，中国科学院院士，发展中国家科学院院士。现任中国科学院党组成员、副秘书长，兼任科技创新发展中心主任、北京分院院长、动物研究所所长。

　　1996年毕业于东北农业大学，获理学博士学位；1997年进入中国科学院发育生物学研究所博士后流动站，1999年获副研究员任职资格；1999-2002年，法国国家农业科学研究院（INRA）分子发育生物学部博士后、项目组长；2002年加入中国科学院动物研究所，任研究员；2015年当选中国科学院院士。

　　周琪研究员主要从事细胞重编程机制和命运调控、干细胞多能性获得与维持等研究工作，并致力于推动干细胞的转化应用和再生医学的发展。周琪研究员在国际首次利用体细胞成功克隆大鼠，并率先开展灵长类克隆研究，最早获得猴体细胞克隆囊胚；研究组现已建立了包括小鼠、大鼠、猪等多个物种的克隆平台；首次获得完全来自iPS细胞的健康小鼠，证明完全重编程的iPS细胞具有与胚胎干细胞相似的多能性；通过多能性干细胞发育能力评估和基因表达分析发现并明确证实决定哺乳动物（小鼠）干细胞多能性的关键基因决定簇，并探索了其调控机制；证明iPS细胞来源的小鼠具有与胚胎干细胞来源小鼠相同的生理功能但具有致瘤倾向性；在体细胞重编程之外，通过外源因子诱导实现了体细胞跨胚层转分化，首次通过转分化获得具有自我更新及分化能力的神经干细胞；建立了小鼠和大鼠的单倍体胚胎干细胞，并利用基因修饰的单倍体胚胎干细胞成功获得健康成活的转基因小鼠和大鼠，为哺乳动物生殖发育、遗传筛选和转基因研究提供了新的工具；发现孤雌单倍体干细胞经过基因组印记修饰后可以替代精子，从而使两只雌性小鼠也能够产生后代，建立了“同性生殖”的新方法；首次创建了新型的异种杂合二倍体胚胎干细胞，为研究进化上不同物种间性状差异的分子机制和X染色体失活提供了新型的有利工具；合作在体外获得功能性精子；合作从精子RNA角度为研究获得性性状的跨代遗传现象开拓了全新的领域；合作揭示了m6A修饰在促进体细胞重编程为多能性干细胞中的重要作用；针对再生医学和临床转化中对动物模型的需求，研究组致力于动物模型的研发，首次利用CRISPR/Cas系统实现大鼠多基因的快速同时敲除；并利用CRISPR技术首次通过胚胎注射一步获得猪血液病模型，证实了CRISPR技术在大动物中的可应用性；通过优化CRISPR/Cas9技术，不通过种系交配而一步直接获得p53基因突变的食蟹猴模型，为快速制备灵长类疾病模型奠定了基础。研究组还建立了小鼠、大鼠、人等多物种的胚胎干细胞系、孤雌胚胎干细胞系及iPS细胞系，依托于多能性干细胞资源建立了种子资源库，建立了多株临床级别的干细胞，开展了细胞治疗及药物筛选等临床前基础研究及安全性评估等工作。

　　周琪研究员现已在 Cell, Nature，Science，Nature Biotechnology, Cell Stem Cell, PNAS，JBC 等刊物发表SCI论文一百余篇，申请、获得重编程及干细胞技术发明专利9项。成果曾入选美国《时代周刊》评选的年度医学十大突破，本人及3项成果先后分别入选年度中国十大科技新闻人物/中国基础研究十大新闻/两院院士评选的十大科技进展/中国科学十大进展。获得国家自然科学二等奖，中科院杰出科技成就奖，何梁何利基金科学与技术进步奖，谈家桢生命科学创新奖，周光召基金会杰出青年基础科学奖，中国青年科技奖，genOway国际转基因技术奖等多项奖励。

(#为共同第一作者，*为通讯作者)

1. Li X#, Cui XL#, Wang JQ#, Wang YK, Li YF, Wang LY, Wan HF, Li TD, Feng GH, Shuai L, Li ZK, Gu Q, Hao J, Wang L, Zhao XY, Liu ZH, Wang XJ, Li W*, Zhou Q*. (2016) Generation and application of mouse-rat allodiploid embryonic stem cells. Cell 164(1-2):279–292.
2. Li Z#, Wan H#, Feng G#, Wang L#, He Z, Wang Y, Wang XJ, Li W*, Zhou Q*, Hu B*. (2016) Birth of fertile bimaternal offspring following intracytoplasmic injection of parthenogenetic haploid embryonic stem cells. Cell Res. 26(1):135-138.
3. Zhou Q#, Wang M#, Yuan Y#, Wang X, Fu R, Wan H, Xie M, Liu M, Guo X, Zheng Y, Feng G, Shi Q, Zhao XY*, Sha J*, Zhou Q* (2016) Complete Meiosis from Embryonic Stem Cell-Derived Germ Cells In Vitro. Cell Stem Cell18(3):330-340.
4. Chen Q#*, Yan M#, Cao Z#, Li X#, Zhang Y#, Shi J#, Feng GH, Peng H, Zhang X, Zhang Y, Qian J, Duan E*, Zhai Q*, Zhou Q*. (2016) Sperm tsRNAs contribute to intergenerational inheritance of an acquired metabolic disorder. Science 351(6271):397-400.
5. Wan H#, Feng C#, Teng F#, Yang S#, Hu B, Niu Y, Xiang AP, Fang W, Ji W, Li W*, Zhao X*, Zhou Q*. (2015) One-step generation of p53 gene biallelic mutant Cynomolgus monkey via the CRISPR/Cas system. Cell Res. 25(2):258-261.
6. Chen Y#, Niu Y#, Li Y#, Ai Z, Kang Y, Shi H, Xiang Z, Yang Z, Tan T, Si W, Li W, Xia X, Zhou Q*, Ji W*, Li T*. (2015) Generation of cynomolgus monkey chimeric fetuses using embryonic stem cells. Cell Stem Cell 17(1):116-124.
7. Chen T#, Hao YJ#, Zhang Y#, Li MM#, Wang M, Han W, Wu Y, Lv Y, Hao J, Wang L, Li A, Yang Y, Jin KX, Zhao X, Li Y, Ping XL, Lai WY, Wu LG, Jiang G, Wang HL, Sang L, Wang XJ*, Yang YG*, Zhou Q*. (2015) m6A RNA methylation is regulated by microRNAs and promotes reprogramming to pluripotency. Cell Stem Cell 16(3):289-301.
8. Li X#, Wang JQ#, Wang LY#, Wan HF, Li YF, Li TD, Wang YK, Shuai L, Mao YH, Cui XL, Wang L, Liu ZH, Li W, Zhou Q*. (2015) Co-participation of paternal and maternal genomes before the blastocyst stage is not required for full-term development of mouse embryos. J Mol Cell Biol. 7(5):486-488.
9. Shuai L#, Wang Y#, Dong M#, Wang X#, Sang L, Wang M, Wan H, Luo G, Gu T, Yuan Y, Feng C, Teng F, Li W, Liu X, Li T, Wang L, Wang XJ, Zhao XY, Zhou Q*. (2015) Durable pluripotency and haploidy in epiblast stem cells derived from haploid embryonic stem cells in vitro. J Mol Cell Biol. 7(4):326-337.
10. Bosley KS, Botchan M, Bredenoord AL, Carroll D, Charo RA, Charpentier E, Cohen R, Corn J, Doudna J, Feng G, Greely HT, Isasi R, Ji W,Kim JS, Knoppers B, Lanphier E, Li J, Lovell-Badge R, Martin GS, Moreno J, Naldini L, Pera M, Perry AC, Venter JC, Zhang F, Zhou Q. (2015) CRISPR germline engineering—the community speaks. Nat. Biotechnol. 33(5):478-486.
11. Andrews PW, Baker D, Benvinisty N, Miranda B, Bruce K, Brüstle O, Choi M, Choi Y-M, Crook JM, de Sousa PA, Dvorak P, Freund C, Firpo M, Furue MK, Gokhale P, Ha H-Y, Han E, Haupt S, Healy L, Hei DJ, Hovatta O, Hunt C, Hwang S-M, Inamdar MS, Isasi RM, Jaconi M, Jekerle V, Kamthorn P, Kibbey MC, Knezevic I, Knowles BB, Koo S-K, Laabi Y, Leopoldo L, Liu P, Lomax GP, Loring JF, Ludwig TE, Montgomery K, Mummery C, Nagy A, Nakamura Y, Nakatsuji N, Oh S, Oh S-K, Otonkoski T, Pera M, Peschanski M, Pranke P, Rajala KM, Rao M, Ruttachuk R, Reubinoff B, Ricco L, Rooke H, Sipp D, Stacey GN*, Suemori H, Takahashi TA, Takada K, Talib S, Tannenbaum S, Yuan B-Z, Zeng F, Zhou Q. (2015) Points to consider in the development of seed stocks of pluripotent stem cells for clinical applications: International Stem Cell Banking Initiative (ISCBI). Regen. Med. 10(2s):1-44.
12. Wang J#, Hao J#, Bai D, Gu Q, Han W, Wang L, Tan Y, Li X, Xue K, Han P, Liu Z, Jia Y, Wu J, Liu L, Wang L, Li W, Liu Z*, Zhou Q*. (2015) Generation of clinical-grade human induced pluripotent stem cells in Xeno-free conditions. Stem Cell Res Ther. 2015 Nov 12;6(1):223. doi: 10.1186/s13287-015-0206-y.
13. Li W#, Li X#, Li T#, Jiang MG#, Wan H, Luo GZ, Feng C, Cui X, Teng F, Yuan Y, Zhou Q, Gu Q, Shuai L, Sha J, Xiao Y, Wang L, Liu Z, Wang XJ, Zhao XY, Zhou Q*. (2014) Genetic modification and screening in rat using haploid embryonic stem cells. Cell Stem Cell 14(3):404-414.
14. Hai T#, Teng F#, Guo R, Li W, Zhou Q*. (2014) One-step generation of knockout pigs by zygote injection of CRISPR/Cas system. Cell Res. 24(3):372-375.
15. Zhou Q*. (2014) Balancing the welfare: the use of non-human primates in research. Trends Genet. 30(11):476-478.
16. Gu Q#, Hao J#, Hai T#, Wang J#, Jia Y, Kong Q, Wang J, Feng C, Xue B, Xie B, Liu S, Li J, He Y, Sun J, Liu L, Wang L, Liu Z*, Zhou Q*. (2014) Efficient generation of mouse ESCs-like pig induced pluripotent stem cells. Protein Cell.5(5):338-342.
17. Li W#, Teng F#, Li T, Zhou Q*. (2013) Simultaneous generation and germline transmission of multiple gene mutations in rat using CRISPR-Cas systems. Nat. Biotechnol. 31(8):684–686.
18. Wan H#, He Z#, Dong M, Gu T, Luo GZ, Teng F, Xia B, Li W, Feng C, Li X, Li T, Shuai L, Fu R, Wang L, Wang XJ, Zhao XY*, Zhou Q*. (2013) Parthenogenetic haploid embryonic stem cells produce fertile mice. Cell Res.23(11):1330-1333.
19. Zhang Y, Teng F, Luo GZ, Wang M, Tong M, Zhao X, Wang L, Wang XJ*, Zhou Q*. (2013) MicroRNA-323-3p regulates the activity of polycomb repressive complex 2 (PRC2) via targeting the mRNA of embryonic ectoderm development (Eed) gene in mouse embryonic stem cells. J Biol. Chem. 288(33):23659–23665.
20. Li W#, Shuai L#, Wan H#, Dong M#, Wang M, Sang L, Feng C, Luo GZ, Li T, Li X, Wang L, Zheng QY, Sheng C, Wu HJ, Liu Z, Liu L, Wang L, Wang XJ, Zhao XY*, Zhou Q*. (2012) Androgenetic haploid embryonic stem cells produce live transgenic mice. Nature 490(7420):407-411. 

    ► 受到Nature China等媒体的报道，并入选2012年度"中国科学十大进展"
    http://www.natureasia.com/en/nchina/article/10.1038/nchina.2012.72

21. Sheng C#, Zheng Q#, Wu J#, Xu Z, Wang L, Li W, Zhang H, Zhao XY, Liu L, Wang Z, Guo C, Wu HJ, Liu Z, Wang L, He S, Wang XJ, Chen Z*, Zhou Q*. (2012) Direct reprogramming of Sertoli cells into multipotent neural stem cells by defined factors. Cell Res. 22(1):208-218. 

    ► 入选中国科学技术信息研究所公布的"2012年中国百篇最具影响国际学术论文"

22. Sheng C#, Zheng Q#, Wu J#, Xu Z, Sang L, Wang L, Guo C, Zhu W, Tong M, Liu L, Li W, Liu ZH, Zhao XY, Wang L, Chen Z*, Zhou Q*. (2012) Generation of dopaminergic neurons directly from mouse fibroblasts and fibroblast-derived neural progenitors. Cell Res. 22(4):769-772.
23. Gu Q#, Hao J#, Zhao XY, Li W, Liu L, Wang L, Liu ZH, Zhou Q*. (2012) Rapid conversion of human ESCs into mouse ESC-like pluripotent state by optimizing culture conditions. Protein Cell. 3(1):71-79.
24. Tong M#, Lv Z#, Liu L#, Zhu H#, Zheng QY#, Zhao XY, Li W, Wu YB, Zhang HJ, Wu HJ, Li ZK, Zeng F, Wang L, Wang XJ, Sha JH*, Zhou Q*. (2011) Mice generated from tetraploid complementation competent iPS cells show similar developmental features as those from ES cells but are prone to tumorigenesis. Cell Res. 21(11):1634-1637. 

    ► 封面文章

25. Li W#, Zhao XY#, Wan HF, Zhang Y, Liu L, Lv Z, Wang XJ, Wang L*, Zhou Q*. (2011) iPS cells generated without c-Myc have active Dlk1-Dio3 region and are capable of producing full-term mice through tetraploid complementation. Cell Res. 21(3):550-553.
26. Riaz A#, Zhao X#, Dai X#, Li W, Liu L, Wan H, Yu Y, Wang L, Zhou Q*. (2011) Mouse cloning and somatic cell reprogramming using electrofused blastomeres. Cell Res. 21(5):770-778.
27. Yu Y#, Yan J#, Liu ZC#, Yan LY, Li M, Zhou Q*, Qiao J*. (2011) Optimal timing of oocyte maturation and its relationship with the spindle assembly and developmental competence of in vitro matured human oocytes. Fertil. Steril. 96(1):73-78.
28. Yu Y#, Zhao C#, Lv Z, Chen W, Tong M, Guo X, Wang L, Liu J, Zhou Z, Zhu H*, Zhou Q*, Sha J. (2011) Microinjection manipulation resulted in the increased apoptosis of spermatocytes in testes from intracytoplasmic sperm injection (ICSI) derived mice. PLoS One 6(7):e22172.
29. Niu Y#, Yu Y#, Bernat A#, Yang S, He X, Guo X, Chen D, Chen Y, Ji S, Si W, Lv Y, Tan T, Wei Q, Wang H, Shi L, Guan J, Zhu X, Afanassieff M, Savatier P*, Zhang K*, Zhou Q*, Ji W*. (2010) Transgenic rhesus monkeys produced by gene transfer into early-cleavage–stage embryos using a simian immunodeficiency virus-based vector. Proc. Natl. Acad. Sci. U. S. A. 107(41):17663-17667. 

    ► 获得中国首例转基因猕猴

30. Zhao XY, Lv Z, Li W, Zeng F*, Zhou Q*. (2010) Production of mice using iPS cells and tetraploid complementation. Nat. Protoc. 5(5):963-971.
31. Liu L#, Luo GZ#, Yang W#, Zhao X#, Zheng Q, Lv Z, Li W, Wu HJ, Wang L, Wang XJ*, Zhou Q*. (2010) Activation of the imprinted Dlk1-Dio3 region correlates with pluripotency levels of mouse stem cells. J. Biol. Chem.285(25):19483-19490. 

    ► 封面文章，并被选为Paper of the Week。被美国生物化学与分子生物学学会(ASBMB)以"Finding is expected to steer future work on therapies down the most efficient and promising paths"为题报道

32. Maruotti J#, Dai XP#, Brochard V, Jouneau L, Liu J, Bonnet-Garnier A, Jammes H, Vallier L, Brons IG, Pedersen R, Renard JP, Zhou Q*, Jouneau A*. (2010) Nuclear transfer-derived epiblast stem cells are transcriptionally and epigenetically distinguishable from their fertilized-derived counterparts. Stem Cells 28(4):743-752.
33. Dai X#, Hao J#, Hou XJ#, Hai T, Fan Y, Yu Y, Jouneau A, Wang L, Zhou Q*. (2010) Somatic nucleus reprogramming is significantly improved by m-carboxycinnamic acid bishydroxamide (CBHA), a histone deacetylase inhibitor. J. Biol. Chem. 285(40):31002-31010.
34. Zhao XY#, Li W#, Lv Z#, Liu L, Tong M, Hai T, Hao J, Guo CL, Ma QW, Wang L, Zeng F*, Zhou Q*. (2009) iPS cells produce viable mice through tetraploid complementation. Nature 461(7260):86-90. 

    ► 2009年入选美国《时代周刊》评选的"十大医学突破"、入选"中国基础研究十大新闻"、两院院士评选的"中国十大科技进展新闻"及"世界十大科技进展新闻"

35. Jin L#, Ji S#, Tang X, Guo X, Lu Y, Chen H, Deng H, Zhou Q*, Ji W*. (2009) Isolation and characterization of liver epithelial progenitor cells from normal adult rhesus monkeys (Macaca mulatta). Cell Res. 19(2):268-270.
36. Yu Y#, Mai Q#, Chen X#, Wang L, Gao L, Zhou C*, Zhou Q*. (2009) Assessment of the developmental competence of human somatic cell nuclear transfer embryos by oocyte morphology classification. Hum. Reprod. 24(3):649-657.
37. Dai XP, Hao J, Zhou Q*. (2009) A modified culture method significantly improves the development of mouse somatic cell nuclear transfer embryos. Reproduction 138(2):301-308.
38. Andrews PW, Arias-Diaz J, Auerbach J, Alvarez M, Ahrlund-Richter L, Baker D, Benvenisty N, Ben-Josef D, Blin G, Borghese L, Borstlap J, Bruce K, Brüstle O, Buckle R, Camby C, Choo A, Chen W, Collins D, Colman A, Crombie C, Crook J, Cypess R, De Sousa P, Dhawan J, Douay L, Dvorak P, Dyke T, Eriksson L, Firpo M, Fitzgerald C, Glover C, Gokhale P, Greene M, Ha HY, Hampl A, Healy L, Hei D, Holm F, Hovatta O, Hunt C, Hwang SM, Inamdar M, Isasi R, Itskovitz-Eldor J, Jessie N, Kim DW, Kirzner R, Kiatpongsan S, Knowles B, Kuo HC, Laughlin M, Lavon N, Ludwig T, Lakov M, Lee DR, Macauley J, McKay R, Menasche P, Menendez P, Michalska A, Mileikovskaia M, Minger S, Mishra G, Moody J, Montgomery K, Morris C, Mummery C, Nagy A, Nakamura Y, Nakatsuji N, Nishikawa S, Oh S, Oh SK, Olson P, Otonkoski T, Patole M, Park HS, Pei X, Pera M, Puceat M, Rajala K, Reubinoff B, Robins A, Rooke H, Rumayor V, Scotman H, Sherlock J, Simon C, Sipp D, Skinner R, Smith D, Stacey G, Stefanovic S, Strehl R, Taft R, Takahashi T, Talib S, Terstegge S, Turner R, Tuuri T, Yu J, Zandstra P, Zapata A, Zeng F, Zhou Q, Tannenbaum S. (2009) Consensus guidance for banking and supply of human embryonic stem cell lines for research purposes. Stem Cell Rev. 5(4):301–314.
39. Ma M#, Guo X#, Wang F, Zhao C, Liu Z, Shi Z, Wang Y, Zhang P, Zhang K, Wang N, Lin M, Zhou Z, Liu J, Li Q, Wang L, Huo R*, Sha J, Zhou Q*. (2008) Protein expression profile of the mouse metaphase-II oocyte. J. Proteome Res.7(11):4821-4830.
40. Wang S#, Shen Y#, Yuan X, Chen K, Guo X, Chen Y, Niu Y, Li J, Xu RH, Yan X*, Zhou Q*, Ji W*. (2008) Dissecting signaling pathways that govern self-renewal of rabbit embryonic stem cells. J. Biol. Chem. 283(51):35929-35940.
41. Niu Y#, Yang S#, Yu Y#, Ding C, Yang J, Wang S, Ji S, He X, Xie Y, Tang X, Zhou Q*, Ji W*. (2008) Impairments in embryonic genome activation in rhesus monkey somatic cell nuclear transfer embryos. Cloning Stem Cells.10(1):25-36.
42. Mai Q#, Yu Y#, Li T, Wang L, Chen MJ, Huang SZ*, Zhou C*, Zhou Q*. (2007) Derivation of human embryonic stem cell lines from parthenogenetic blastocysts. Cell Res. 17(12):1008-1019.
43. Yang J, Yang S, Beaujean N, Niu Y, He X, Xie Y, Tang X, Wang L, Zhou Q*, Ji W*. (2007) Epigenetic marks in cloned rhesus monkey embryos: comparison with counterparts produced in vitro. Biol. Reproduc. 76(1):36-42.
44. Yang S*, He X, Hildebrandt TB, Jewgenow K, Goeritz F, Tang X, Zhou Q*, Ji W*. (2007) Effects of rhFSH dose on ovarian follicular response, oocyte recovery and embryo development in rhesus monkeys. Theriogenology 67(6):1194-1201.
45. Zhou Q#*, Yang SH#, Ding CH#, He XC, Xie YH, Hildebrandt TB, Mitalipov SM, Tang XH, Wolf DP*, Ji WZ*. (2006) A comparative approach to somatic cell nuclear transfer in the rhesus monkey. Hum. Reproduc. 21(10):2564-2571.
46. Li T, Zheng J, Xie Y, Wang S, Zhang X, Li J, Jin L, Ma Y, Wolf DP, Zhou Q*, Ji W*. (2005) Transplantable neural progenitor populations derived from rhesus monkey embryonic stem cells. Stem Cells 23(9):1295-1303.
47. Li T, Wang S, Xie Y, Lu Y, Zhang X, Wang L, Yang S, Wolf D, Zhou Q*, Ji W*. (2005) Homologous feeder cells support undifferentiated growth and pluripotency in monkey embryonic stem cells. Stem Cells 23(8):1192-1199.
48. Zhou Q, Renard JP*, Le Friec G, Brochard V, Beaujean N, Cherifi Y, Fraichard A, Cozzi J. (2003) Generation of fertile cloned rats by regulating oocyte activation. Science 302(5648):1179. 

    ► 受到Nature，Science等杂志评价，并因此获得国际转基因研究genOway奖、入选2003年中国十大科技新闻人物 
    http://www.sciencemag.org/news/2003/09/lab-rats-cloned-finally

49. Zhou Q, Jouneau A, Brochard V, Adenot P, Renard JP*. (2001) Developmental potential of mouse embryos reconstructed from metaphase embryonic stem cell nuclei. Biol. Reproduc. 65(2):412-419.
50. Zhou Q, Boulanger L, Renard JP*. (2000) A simplified method for the reconstruction of fully competent mouse zygotes from adult somatic donor nuclei. Cloning 2(1):35-44. 

    ► 2000年被"克隆羊多莉之父" Wilmut教授评价为"克隆领域迄今为止最重要的文章之一"